Journal: BMC Cancer
Article Title: Comprehensive analysis of alternative polyadenylation regulators concerning CD276 and immune infiltration in bladder cancer
doi: 10.1186/s12885-022-10103-7
Figure Lengend Snippet: Analysis of the CPSF3 gene expression in BC. A The Venn diagram suggested that the upregulation of CPSF3 was an unfavorable prognostic factor and positively correlated with the CD276 expression in bladder cancer (BC). B The heat map visualized the relative expression of 26 APA regulators in BC/normal (row 1) and the correlations of 26 regulators with the CD276 expression (row 2) and prognosis (row 3). The red color indicates a high expression in BC, positively correlated with CD276 or unfavorable prognosis. * p < 0.05 and ** p < 0.01. CPSF3 was upregulated in BC tissues compared to normal tissues.TCGA-BLCA cohort ( C ), GSE13507 ( D ), and GSE38264 ( E ). F Relative expression of CPSF3 was detected by qRT -PCR in 16 pairs of BC and normal tissues. G Compared with SV-HUC-1 cell line (normal), CPSF3 was highly expressed in EJ, T24, TCCSUP, UMUC3, 5637, RT4 cell lines (BC). H Immunohistochemistry staining indicated that, compared with normal bladder tissue (left), CPSF3 was significantly elevated in BC tissue (right) in the human protein atlas (antibody HPA034657, × 10). * p < 0.05, ** p < 0.01
Article Snippet: The protein expression level of CPSF3 in BC compared to normal tissue was obtained from the Human Protein Atlas database ( https://www.proteinatlas.org/ ).
Techniques: Gene Expression, Expressing, Quantitative RT-PCR, Immunohistochemistry, Staining